Cbs-scientific QNX-700 Instrukcja Użytkownika

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Strona 1 - INSTRUCTION MANUAL

Quadra Electrophoresis Systems4-Place Mini-Vertical Slab Gel /Blotting SystemsQNC-700, with coolingQNX-700INSTRUCTION MANUAL

Strona 2 - TABLE OF CONTENTS

10Quadra Instructions 9-30-133.2 Running the Gel1. Place stirring bar into bottom of reservoir in stirring corral.2. Place core assemblies into lower

Strona 3 - IMPORTANT USER INFORMATION

11www.cbsscientic.com793.3 Removing the Gel1. Turn the power supply off and discon-nect the leads from the power supply. Remove the safety cover fro

Strona 4 - Quadra Instructions 9-30-13

12Quadra Instructions 9-30-134.1 Alternate Protocol: Using Gel Wrap™ Gasket Casting Method (Not using Pre-cast gels)1. Place all components in an a

Strona 5 - SECTION 1

13www.cbsscientic.com4.1 Alternate Protocol: Using Gel Wrap™ Gasket Casting Method (Not using Pre-cast gels)1. Place all components in an authoriz

Strona 6 - 1.4 Safety

14Quadra Instructions 9-30-13SECTION 4 Alternate Protocol for Slab Gel Electrophoresis. (Not using Pre-cast gels)4.2 Preparing the Electrophoresis U

Strona 7 - Description of Parts

15www.cbsscientic.com1SECTION 5 Instructions for Western Blotting5.1 Preparing the Unit for Blotting1. Remove safety cover from the assembled unit b

Strona 8 - SECTION 3

16Quadra Instructions 9-30-134. Insert blotting casettes into core making sure that red side faces outward. See diagram 5.5. Close doors and re-latch

Strona 9

17www.cbsscientic.com4. Insert blotting casettes into core making sure that red side faces outward. See diagram 5.5. Close doors and re-latch by pres

Strona 10 - 3.2 Running the Gel

18Quadra Instructions 9-30-136.2 Electro-Blotting6.3 References1. Hames, B.D. (ed.) (1998). Gel Electrophoresis of Proteins. A Practical Approach.

Strona 11 - 3.3 Removing the Gel

19www.cbsscientic.comAs a general recommendation, equilibrate gels (after running) with the diluted transfer buffer for 5 to 10 minutes before transf

Strona 12 - (Not using Pre-cast gels)

2Quadra Instructions 9-30-13TABLE OF CONTENTS Important User Information . . . . . . .

Strona 13

20Quadra Instructions 9-30-136.4 Recommended ClearPAGE™ Buffer Formulations, con’t.LDS Sample Buffer, 4x, (Cat. # FB31010) *pH should be betwee

Strona 14 - SECTION 4

21www.cbsscientic.comSECTION 7Maintenance of Equipment7.1 Care and Handling The plastic components of the Quadra Electrophoresis Systems are fabrica

Strona 15 - SECTION 5

22Quadra Instructions 9-30-13SECTION 8Ordering Information CAT. # DESCRIPTIONQUADRA COMBO MINI-VERTICALS - 10CM(W) X 10CM(H)*QNX-700Quadra System

Strona 16 - 5.3 Removing the Blot

23www.cbsscientic.comSECTION 8Ordering InformationClearPAGE SDS gels, packages of 10 - Cat. #’sAcrylamide %Gel Size (cm) W x H1 wellCat. #2D well tro

Strona 17 - 6.1.3 Tris-Glycine Gels

CONTACT INFORMATIONOnline Ordering www.cbsscientic.comTelephone Local and International: 858-755-4959 Toll Free: 800-243-4959Sales E-mail Address

Strona 18 - 6.2 Electro-Blotting

3www.cbsscientic.comIMPORTANT USER INFORMATIONThis Instruction Manual will explain how to use this product safely and effectively. Please read and c

Strona 19 - SDS (2%) 288.38 - 20.0 g

4Quadra Instructions 9-30-13FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURSLe présent manuel d’utilisation explique la manière de se serv

Strona 20 - , (Cat. # GB33002)

5www.cbsscientic.comSECTION 1General Information 1.1 IntroductionC.B.S. Scientic offers the Quadra Electrophoresis Systems for performing SDS-Pag

Strona 21 - Maintenance of Equipment

6Quadra Instructions 9-30-131.3 Comb Specications for Hand Cast Gels1.4 SafetyPower to the Quadra Electrophoresis System is to be supplied by an

Strona 22 - CAT. # DESCRIPTION

7www.cbsscientic.comSECTION 2 Description of Parts2.1 Unpacking and Components Please verify that your unit comes complete with the following comp

Strona 23 - Ordering Information

8Quadra Instructions 9-30-13SECTION 3 Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels3.1 Preparing the Electrophoresis Unit1. Power le

Strona 24 - CONTACT INFORMATION

9www.cbsscientic.comSECTION 3 Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels3.1 Preparing the Electrophoresis Unit1. Power leads are

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